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bases will not match, hybridization can usually be facilitated by adjusting the pH or ionic strength of the solution. The hybridized oligonucleotide is then used as a primer for DNA replication; the whole plasmid is replicated, resulting in hybrid DNA. In subsequent DNA replications of the hybrid, both the original gene and the mutated DNA will be produced. The latter can be isolated by appropriate selection methods; it is a plasmid with a cloned gene that has the exact mutation the researcher wanted. Using techniques of this type, geneticists have made many advancements in understanding exactly how various components of an enzyme contribute to its function.

VALUE() is used to convert text into a number It accepts both literals and column references It also works against dates The text, of course, must be a valid number 123 will work while x123 will not The examples are Products table calculated columns

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In these instructions, the byte that was pushed down onto the stack with 2 bytes on top of it is accessed by adding three to the stack pointer and storing the value in the w register (destroying the offset to the byte put there earlier). This capability makes the PIC18 a very powerful architecture to work with and allows you and compiler writers to develop code that is similar to what is used on high-end processors. There is one method of passing parameters to and from that I haven t discussed because I do not believe that it is an appropriate method for the PIC microcontroller, and that is using the processor s registers to store parameters. For the PIC microcontroller, there is only the w register, which is 8 bits wide, that can be guaranteed for the task. To frustrate using this method, the low-end devices lack of a return instruction prevents passing data back using the w register except in the case of using the retlw instruction and a jump to a table offset. The zero, carry, and digit carry STATUS register ags also could be used for this purpose, and they are quite effective for being used as pass/fail return ags.

Figure 2 Site-directed mutagenesis can involve any nucleotide(s). In this case,

This section was originally titled Working with the Architecture s Quirks because there are some unusual features about the architecture that make copying assembly-language applications directly from another microcontroller to the PIC microcontroller dif cult. However, as I started listing what I wanted to do in this and the following sections, I realized that there were many advantages to the PIC microcontroller s architecture and that many of the quirks actually allow very ef cient code to be written for different situations. In this and the following sections I will discuss how the PIC microcontroller architecture can be used to produce some code that is best described as funky. In addition, the basic operation sequence of adding two numbers together is

1 Load the accumulator with the rst additional RAM. 2 Add the second additional RAM to the contents of the accumulator. 3 Store the contents of the accumulator into the destination.

an inserted gene with an Ile-Gly sequence is converted, at the direction of the investigator, to an Ile-Ala sequence. A single-stranded form of the plasmid is isolated. A synthetically prepared oligonucleotide (twenty-three bases in this example) is added. It can be made to hybridize at the complementary site despite differing by three bases. Then DNA replication is carried out using the oligonucleotide con guration as a primer. After the strands of the duplex are separated, the investigators can isolate the original plasmid as well as the mutated plasmid. (Note that the investigators changed two codons, although they changed only one amino acid, because they also wanted to introduce an Alu site at that point for future studies.) (From J. E. Villafranca, et al., Directed mutagenesis of dihydrofolate reductase, Science 222:782 88. Copyright 1983 by the AAAS.)

If it s required, the movf and addwf instructions can be changed to movlw or addlw, respectively, if either parameter is a constant. Subtraction in the PIC microcontroller follows a similar set of instructions, but because of the way the subtraction operation works, the subtracted value must be loaded rst into the accumulator. For example, for the high level language statement

The McGraw Hill Companies, 2001

=VALUE(LEFT('Products'[QuantityPerUnit],1)) =VALUE(LEFT('Products'[QuantityPerUnit],2))

from the rst).

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